Revalidation of Passalites Gloger, 1841 for the Amazon brown brocket deer P.nemorivagus (Cuvier, 1817) (Mammalia, Artiodactyla, Cervidae).

Mazamanemorivaga (Cuvier, 1817) is a gray brocket deer that inhabits the Amazon region. An assessment of previous studies revealed inconsistencies in its current taxonomic classification, suggesting the need for an update in its genus classification. A taxonomic repositioning of this species is proposed through the collection of a specimen from its type locality (French Guiana) with subsequent morphological (coloring pattern, body measurements, and craniometry), cytogenetics (G Band, C Band, conventional Giemsa, Ag-NOR staining, and BAC probe mapping), and molecular phylogenetic analysis (mitochondrial genes Cyt B of 920 bp, COI I of 658 bp, D-loop 610 bp), and comparisons with other specimens of the same taxon, as well as other Neotropical deer species. The morphological and cytogenetic differences between this and other Neotropical Cervidae confirm the taxon as a unique and valid species. The phylogenetic analysis evidenced the basal position of the M.nemorivaga specimens within the Blastocerina clade. This shows early diversification and wide divergence from the other species, suggesting that the taxon should be transferred to a different genus. A taxonomic update of the genus name is proposed through the validation of Passalites Gloger, 1841, with Passalitesnemorivagus (Cuvier, 1817) as the type species. Future research should focus on evaluating the potential existence of other species within the genus Passalites, as suggested in the literature.

The emotional impact of COVID-19 on Spanish nurses and potential strategies to reduce it.

Background: Although the emotional and psychological impact of nurses' work had been identified before the COVID-19 pandemic, the pandemic aggravated risk indicators for their mental health. Aim: The objective of this study was to analyse the levels of anxiety, depression, post-traumatic stress and burnout of nurses in the Balearic Islands (Spain) during the pandemic to identify possible sociodemographic and related occupational factors. Design: A cross-sectional study of 892 nurses was conducted during four weeks from February to March 2021. Methods: Sociodemographic data related to the pandemic were collected and anxiety, depression, burnout and post-traumatic stress were measured with validated scales. A multivariate and predictive analysis was carried out with risk estimates. Findings: About 75.6% of the nurses had experience in COVID-19 units, and 49.1% had worked for more than 10 months in a COVID-19 unit. Nurses in COVID-19 units (hospital ward or ICU) were more likely to report emotional fatigue (OR 1.9, p < 0.001) and anxiety (OR 1.5, p = 0.021). In general, moderate post-traumatic stress was evident in general nurses (p = 0.027), and severe post-traumatic stress was evident in ICU nurses (p = 0.027). A 1.24-month reduction in COVID-19 patient care predicted reduced levels of emotional fatigue (5.45 points), depersonalisation (1.87 points) and post-traumatic stress (4.65 points) in nurses. Conclusion: Given the occurrence of new waves of COVID-19, the need to establish preventive strategies that focus on the personal and occupational characteristics related to these indicators and to implement urgent psychological support strategies is demonstrated. Impact: Given these findings, it is imperative solutions are urgently applied in order to prevent compounding risk to the health system.

Designation of a neotype for Mazama americana (Artiodactyla, Cervidae) reveals a cryptic new complex of brocket deer species.

Mazama americana (red brocket deer) is the genus-type species (first species described for this genus) and the basis for the identity of other Mazama species. Mazama americana is one of the most abundant and widely distributed deer species in the neotropical forest. However, recent studies suggest that this taxon belongs to a species complex. Our goal was to collect an animal at the type locality (topotype) in French Guiana with the aim of characterizing the morphological (biometric, craniometric), cytogenetic (Giemsa, C-banding, G-banding and NOR) and molecular (mitochondrial DNA) features. The comparisons showed that the collected specimen was very similar morphologically to specimens from other South American populations, but it was cytogenetically and molecularly very different from any of the cytotypes already described for this species, corroborating the existence of a complex of cryptic species. The data suggest that the M. americana topotype is a different species from all the cytotypes already described in the literature and which occupy the southern region of the Amazon River. The characterization and designation of the M. americana neotype is the first step toward a taxonomic reorganization of the genus Mazama, with the potential identification of new species.

Cooperation as a solution to shared resources in territorial use rights in fisheries.

Territorial use rights in fisheries (TURFs) are coastal territories assigned to fishermen for the exclusive extraction of marine resources. Recent evidence shows that the incentives that arise from these systems can improve fisheries sustainability. Although research on TURFs has increased in recent years, important questions regarding the social and ecological dynamics underlying their success remain largely unanswered. In particular, in order to create new successful TURFs, it is critical to comprehend how fish movement over different distances affects the development of sustainable fishing practices within a TURF. In theory, excessive spillover outside a TURF will generate incentives to overharvest. However, many TURFs have proven successful even when targeted species move over distances far greater than the TURF's size. A common attribute among some of these successful systems is the presence of inter-TURF cooperation arrangements. This raises the question of how different levels and types of cooperation affect the motivations for overharvesting driven by the movement of fish outside the TURF. In this paper, we examine equilibrium yields under different levels of inter-TURF cooperation (from partial to full) and varying degrees of asymmetry across TURFs of both biological capacity and benefit-sharing. We find that partial cooperation can improve yields even with an unequal distribution of shared benefits and asymmetric carrying capacity. However, cooperation arrangements are unstable if the sharing agreement and biological asymmetries are misaligned. Remarkably, we find that asymmetry in the system can lead to the creation of voluntary no-take zones.

Genetic diversity and hematological and biochemical alterations in Alouatta primates naturally infected with hemoplasmas in Brazil.

Mycoplasma spp. and Bartonella spp. are Gram-negative bacteria transmitted by arthropod vectors that infect red blood cells of several mammal species. This study investigated the occurrence and genetic diversity of hemoplasmas and Bartonella spp. in 68 howler monkeys kept in captivity in São Paulo, a southeastern state in Brazil. In addition, possible hematological, biochemical and electrophoretic changes of serum proteins associated with the occurrence of hemoplasmas and Bartonella spp. in captive primates were also investigated. The cPCR results showed that all sampled howler monkeys were negative for Bartonella spp. based on the gltA gene. The cPCR results indicated that 18 (26.47%) non-human primates (NHP) were positive for hemoplasmas based on the 16S rRNA gene. Monocyte and lymphocyte counts were higher in hemoplasma-positive howlers (P < 0.05). Platelet counts decreased in nonhuman primates (NHP) positive for hemoplasmas (P < 0.05). The results from the blood serum proteinogram and biochemistry analyses were not significantly different between NHPs positive and negative for hemotrophic mycoplasmas. Phylogenetic analysis using Bayesian Inference (BI) based on the 16S rRNA gene positioned the obtained sequences close to 'Candidatus Mycoplasma kahanei'. The analysis of sequence diversity of the 16S rRNA gene showed that 5 different genotypes are circulating in NHP in Brazil and in the world; besides, a clear separation between the sequences of hemoplasmas that infect NHP of the Sapajus and Alouatta genus in Brazil was found, probably corresponding to two different species. The pathogenic potential of this hemoplasma species in NHP should be further investigated.

Correction: Eco-Label Conveys Reliable Information on Fish Stock Health to Seafood Consumers.

[This corrects the article DOI: 10.1371/journal.pone.0043765.].

Effects of fish movement assumptions on the design of a marine protected area to protect an overfished stock.

Marine Protected Areas (MPA) are important management tools shown to protect marine organisms, restore biomass, and increase fisheries yields. While MPAs have been successful in meeting these goals for many relatively sedentary species, highly mobile organisms may get few benefits from this type of spatial protection due to their frequent movement outside the protected area. The use of a large MPA can compensate for extensive movement, but testing this empirically is challenging, as it requires both large areas and sufficient time series to draw conclusions. To overcome this limitation, MPA models have been used to identify designs and predict potential outcomes, but these simulations are highly sensitive to the assumptions describing the organism's movements. Due to recent improvements in computational simulations, it is now possible to include very complex movement assumptions in MPA models (e.g. Individual Based Model). These have renewed interest in MPA simulations, which implicitly assume that increasing the detail in fish movement overcomes the sensitivity to the movement assumptions. Nevertheless, a systematic comparison of the designs and outcomes obtained under different movement assumptions has not been done. In this paper, we use an individual based model, interconnected to population and fishing fleet models, to explore the value of increasing the detail of the movement assumptions using four scenarios of increasing behavioral complexity: a) random, diffusive movement, b) aggregations, c) aggregations that respond to environmental forcing (e.g. sea surface temperature), and d) aggregations that respond to environmental forcing and are transported by currents. We then compare these models to determine how the assumptions affect MPA design, and therefore the effective protection of the stocks. Our results show that the optimal MPA size to maximize fisheries benefits increases as movement complexity increases from ~10% for the diffusive assumption to ~30% when full environment forcing was used. We also found that in cases of limited understanding of the movement dynamics of a species, simplified assumptions can be used to provide a guide for the minimum MPA size needed to effectively protect the stock. However, using oversimplified assumptions can produce suboptimal designs and lead to a density underestimation of ca. 30%; therefore, the main value of detailed movement dynamics is to provide more reliable MPA design and predicted outcomes. Large MPAs can be effective in recovering overfished stocks, protect pelagic fish and provide significant increases in fisheries yields. Our models provide a means to empirically test this spatial management tool, which theoretical evidence consistently suggests as an effective alternative to managing highly mobile pelagic stocks.

Eco-label conveys reliable information on fish stock health to seafood consumers.

Concerns over fishing impacts on marine populations and ecosystems have intensified the need to improve ocean management. One increasingly popular market-based instrument for ecological stewardship is the use of certification and eco-labeling programs to highlight sustainable fisheries with low environmental impacts. The Marine Stewardship Council (MSC) is the most prominent of these programs. Despite widespread discussions about the rigor of the MSC standards, no comprehensive analysis of the performance of MSC-certified fish stocks has yet been conducted. We compared status and abundance trends of 45 certified stocks with those of 179 uncertified stocks, finding that 74% of certified fisheries were above biomass levels that would produce maximum sustainable yield, compared with only 44% of uncertified fisheries. On average, the biomass of certified stocks increased by 46% over the past 10 years, whereas uncertified fisheries increased by just 9%. As part of the MSC process, fisheries initially go through a confidential pre-assessment process. When certified fisheries are compared with those that decline to pursue full certification after pre-assessment, certified stocks had much lower mean exploitation rates (67% of the rate producing maximum sustainable yield vs. 92% for those declining to pursue certification), allowing for more sustainable harvesting and in many cases biomass rebuilding. From a consumer's point of view this means that MSC-certified seafood is 3-5 times less likely to be subject to harmful fishing than uncertified seafood. Thus, MSC-certification accurately identifies healthy fish stocks and conveys reliable information on stock status to seafood consumers.

Exposición ocupacional al calor en una empresa refinadora de metales / Occupational heat exposure in a metal industry

El estudio va dirigido a evaluar algunos indicadores ambientales y biológicos de un grupo de trabajadores expuestos a calor ambiental en razón de la actividad laboral que desempeñan. En ellos, se determina la carga calórica, gasto energético, temperatura corporal y la energía disponible de los trabajadores para realizar su trabajo. Mediante un procedimiento observacional y analítico se evaluaron en relación a las normas habituales establecidas para dichos efectos 2 puestos de trabajo, y en base a ellos se exponen los procedimientos utilizados, los resultados obtenidos y los alcances que los autores estiman de importancia desde un punto de vista ergonómico